I-PACAP 1-27 CAS: 127317-03-7 i-pituitary adenylate cyclase activating*polypeptid

I-Neuropituitrin iyi-vasoconstrictor kanye ne-antidiuretic, engasetshenziselwa i-postpartum hemostasis, ekhuthaza ukufinyela kwesibeletho kanye nokukhipha isisu okungaphelele.Iphinde isetshenziselwe ekwelapheni esophageal kanye esiswini fundus varices rupture ukopha, isifo sikashukela insipidus nokunye.Izindlela zokukhiqiza Ukukhishwa kwempushana yeqabunga le-pituitary ngemuva Iqabunga elingemuva lomiswa endaweni yokomisa nge-phosphorus pentoxide (umswakama ongaphansi kuka-3%) futhi ligaywe libe yimpushana ngomshini wokugaya ukuze kutholwe impushana yeqabunga engemuva.Bese wengeza i-acetone engu-90% (izikhathi ezi-3-6), gudluza ku-25℃ amahora angu-4 bese uhamba ubusuku bonke.Ngakho-ke, ukwelashwa okuphindaphindiwe izikhathi ezingu-2-3, thatha inani elincane loketshezi lwe-acetone kanye nenani elincane lamanzi acwengekile alisekho i-turbidity.Bese iphathwa nge-ether (izikhathi ezi-3 inani) isikhathi esingu-1, ihlungiwe, i-ether volatilization yemvelo ukuze yome, etholwe ngemva kwempushana yeqabunga.I-acetone, ukwelashwa kwe-ether ukususa izinto eziphansi zamangqamuzana nama-lipids, ukuqinisekisa ukucaca.Ingulube ye-porcine neurohypophysial [P2O5]→ i-hypophysial powder engahluziwe [i-acetone, i-ether]→[25℃,4h] Ukuhlanzwa kwe-hypophysial powder I-hypophysial powder ikhishwe kabili ngokungeza i-0.25% i-acetic acid, okokuqala yengeza izikhathi ezingu-5 inani, okwesibili wengeza izikhathi ezi-2 inani, ugqugquzela ku-50 ℃ i-15min, ukuhlunga.Ngemva kokuqedwa kokukhishwa okubili, i-filtrate yahlanganiswa futhi yashiswa ngesivinini esisheshayo se-Chemicalbook kuya ku-100 ℃ nge-15min.Bese kuthi u-0.5% we-trichloro-tert-butanol (isilinganiso sevolumu engu-1:1, ukwelashwa kwekhabhoni ecushiwe uma kunesidingo) kwengezwe ngenkathi kushisa.I-filtrate yapholiswa ngezinga elisheshayo laya ngaphansi kuka-30 ℃.Ukukhishwa kwe-acetic acid kuqukethe amaprotheni angasebenzi kanye nama-enzyme azi-hydrolyzing, angase ahlanganiswe futhi abhujiswe ngokushisa (qaphela: i-oxytocin titer ye-oxytocin kulula ukwehluleka ngaphansi kwe-alkaline noma ama-asidi aqinile. Izinzile kuma-asidi abuthakathaka njenge-pH3-4, futhi inzalo yesitimu ku-100℃ imizuzu engama-30 noma ukuvala inzalo yokucindezela okushisayo nakho kuzinzile).Impushana yeqabunga le-post-pituitary [0.25% acetic acid]→[50℃,15min] filtrate [0.5% trichloro-tert-butanol]→[esiqandisi, ehlungiwe] impushana yeqabunga engemuva kokuhlanjululwa;impushana yeqabunga ehlanjululwe ngemuva;impushana yeqabunga ehlanjululwe ngemuva;impushana yeqabunga ehlanjululwe ngemuva;impushana yeqabunga ehlanjululwe ngemuva;impushana yeqabunga ehlanjululwe ngemuva;impushana yeqabunga ehlanjululwe ngemuva;impushana yeqabunga ehlanjululwe ngemuva;impushana yeqabunga ehlanjululwe ngemuva;impushana yeqabunga ehlanjululwe ngemuva;I-pH yalungiswa yaba ngu-3.5 nge-glacial acetic acid, yahlungwa ngefaneli encibilikayo eqondile, ivalwe ngebhodwe, futhi yafakwa inzalo ngesitimu esingu-100℃ isigamu sehora ukuze kutholwe umjovo we-pituitary wangemuva.Impushana ye-Afterleaf [ukuhlolwa kwe-titer]→[pH3.5] Umjovo we-Afterleaf [ukuhlunga, ukuvala inzalo]→ umjovo we-afterleaf oqediwe.