PACAP 1-27 CAS: 127317-03-7 pituitary adenylate cyclase activating*polypeptid

I-Neuropituitrin i-vasoconstrictor kunye ne-antidiuretic, engasetyenziselwa i-postpartum hemostasis, ukukhuthaza ukunyanzeliswa kwe-uterine kunye nokukhipha isisu okungaphelelanga.Ikwasetyenziselwa unyango lwe-sophageal kunye ne-gastric fundus varices rupture ukopha, isifo seswekile insipidus njalo njalo.Iindlela zokuvelisa Ukutsalwa komgubo wegqabi ongemva kwepituitary Igqabi elingasemva lomiswa kwivacuum dryer ene-phosphorus pentoxide (ubufuma obungaphantsi kwe-3%) kwaye yagutywa ibe ngumgubo ngebhola yokusila ukufumana umgubo orhabaxa wamagqabi angasemva.Emva koko faka i-90% ye-acetone (i-3-6 amaxesha), gxuma kwi-25 ℃ ye-4h kwaye ushiye ubusuku bonke.Ngoko unyango oluphindaphindiweyo ngamaxesha angama-2-3, thatha inani elincinane le-acetone engamanzi kunye nenani elincinci lamanzi adibeneyo akusekho i-turbidity.Emva koko uphathwe nge-ether (i-3 amaxesha inani) ixesha le-1, ihluzo, i-ether volatilization yendalo ukuze yome, efunyenwe emva komgubo weqabunga.I-acetone, unyango lwe-ether kukususa izinto eziphantsi ze-molecular kunye ne-lipids, ukuqinisekisa ukucaca.I-porcine neurohypophysial [P2O5]→ i-hypophysial powder engcolileyo [i-acetone, i-ether]→[25℃,4h] Ukuhlanjululwa kwe-hypophysial powder I-powder ye-hypophysial ikhutshwe kabini ngokudibanisa i-0.25% i-acetic acid, okokuqala yongeza amaxesha ama-5 isixa, okwesibini. ukongeza amaxesha ama-2 inani, uvuselela kwi-50 ℃ ye-15min, ukuhluza.Emva kokuba izicatshulwa ezimbini zigqityiwe, i-filtrate yadityaniswa kwaye yafudumala ngesantya esikhawulezayo se-Chemicalbook ukuya kwi-100 ℃ ye-15min.Emva koko i-0.5% i-trichloro-tert-butanol (i-1: i-1: i-volume ratio, i-activated carbon treatment ukuba kuyimfuneko) yongezwa ngelixa ishushu.Isihluzi sapholiswa ngesantya esikhawulezayo ukuya ngaphantsi kwe-30℃.I-acetic acid extract iqulethe iiprotheni ezingasebenziyo kunye ne-self-hydrolyzing enzymes, enokuthi idibanise kwaye itshatyalaliswe ngokufudumeza (qaphela: i-oxytocin titer ye-oxytocin kulula ukusilela phantsi kwe-alkaline okanye i-acids eqinile. Izinzile kwii-acids ezibuthathaka ezifana ne-pH3-4, kwaye ukuvala umphunga kwi-100℃ nge-30min okanye ukuvala inzala yoxinzelelo olushushu nako kuzinzile).I-Post-pituitary leaf powder [0.25% acetic acid]→[50℃,15min] filtrate [0.5% trichloro-tert-butanol]→[ifriji, ihluziwe] umgubo wamagqabi ocociweyo emva kokucocwa;umgubo weqabunga emva kokuhlanjululwa;umgubo weqabunga emva kokuhlanjululwa;umgubo weqabunga emva kokuhlanjululwa;umgubo weqabunga emva kokuhlanjululwa;umgubo weqabunga emva kokuhlanjululwa;umgubo weqabunga emva kokuhlanjululwa;umgubo weqabunga emva kokuhlanjululwa;umgubo weqabunga emva kokuhlanjululwa;umgubo weqabunga emva kokuhlanjululwa;I-pH yahlengahlengiswa ukuya kwi-3.5 nge-acetic acid enomkhenkce, yahluzwa ngefaneli yokunyibilika ethe nkqo, imbiza etywiniweyo, yaza yafakwa inzala ngomphunga kwi-100℃ kangangesiqingatha seyure ukufumana inaliti yepituitary yangasemva.Umgubo weAfterleaf [uvavanyo lwetiter]→[pH3.5] Isitofu sasemva kwegqabi [ukuhluzwa, ukuvala inzala]→ naliti yasemva kwegqabi imveliso egqityiweyo.