PACAP 1-27 CAS: 127317-03-7 pituitary adenylate cyclase activating * polypeptid

Neuropituitrin ke vasoconstrictor le antidiuretic, e ka sebelisoang bakeng sa hemostasis ea postpartum, e khothalletsang ho ferekana ha uterine le ho ntša mpa ho sa phethoang.E boetse e sebelisoa bakeng sa phekolo ea esophageal le gastric fundus varices rupture blood, lefu la tsoekere insipidus joalo-joalo.Mekhoa ea tlhahiso Ho ntšoa ha phofshoana ea lekhasi la pituitary ka morao Lekhasi le ka morao le ile la omisoa ka har'a mochini o omisang metsi ka phosphorus pentoxide (mongobo o ka tlase ho 3%) le ho siloa ho ba phofo ka leloala la bolo ho fumana phofo e mahoashe ea makhasi a ka morao.Ebe u eketsa 90% acetone (makhetlo a 3-6), tsosa ka 25 ℃ bakeng sa 4h 'me u tsamaee bosiu bo le bong.Ka hona, kalafo e pheta-phetoang makhetlo a 2-3, nka mokelikeli o monyane oa acetone le metsi a mangata a silafetseng ha e sa le turbidity.Ebe e tšoaroa ka ether (makhetlo a 3 chelete) bakeng sa nako ea 1, e tlhotliloeng, ether ea tlhaho ea tlhaho ho omella, e fumanoang ka mor'a phofo ea makhasi.Acetone, kalafo ea ether ke ho tlosa lintho tse tlase tsa limolek'hule le lipids, ho netefatsa ho hlaka.Porcine neurohypophysial [P2O5]→ crude hypophysial powder [acetone, ether]→[25℃,4h] Ho hloekisoa ha phofo ea hypophysial Phofo ea hypophysial e ile ea ntšoa ka makhetlo a mabeli ka ho eketsa 0.25% acetic acid, lekhetlo la pele ho eketsa makhetlo a 5 palo, lekhetlo la bobeli eketsa makhetlo a 2 palo, e susumetsang ka 50 ℃ bakeng sa 15min, filtration.Ka mor'a hore lihlahisoa tse peli li phethoe, filtrate e ile ea kopanngoa 'me ea futhumatsoa ka lebelo le potlakileng la Chemicalbook ho 100 ℃ bakeng sa 15min.Ebe 0.5% trichloro-tert-butanol (1: 1 bophahamo ba molumo, activated carbon treatment haeba ho hlokahala) e ile ea eketsoa ha ho chesa.Filtrate e ile ea pholile ka lebelo le potlakileng ho ea ka tlase ho 30 ℃.Setlolo sa acetic acid se na le liprotheine tse sa sebetseng le li-enzyme tse iketsetsang metsi, tse ka kopanngoang le ho senngoa ke ho futhumatsa (hlokomela: oxytocin titer ea oxytocin e bonolo ho hlōleha tlas'a alkaline kapa acid e matla. E tsitsitseng ka acid e fokolang e kang pH3-4, le sterilization ea mouoane ho 100 ℃ bakeng sa 30min kapa ho thibela ho chesa ha khatello e chesang le hona ho tsitsitse).Phofo ea lekhasi la post-pituitary [0.25% acetic acid]→[50℃,15min] filtrate [0.5% trichloro-tert-butanol]→[e entsoeng ka sehatsetsing, e tlhotliloeng] phofshoana ea lekhasi e hloekisitsoeng ka morao;phofshoana ea lekhasi e hloekisitsoeng ka mor'a;phofshoana ea lekhasi e hloekisitsoeng ka mor'a;phofshoana ea lekhasi e hloekisitsoeng ka mor'a;phofshoana ea lekhasi e hloekisitsoeng ka mor'a;phofshoana ea lekhasi e hloekisitsoeng ka mor'a;phofshoana ea lekhasi e hloekisitsoeng ka mor'a;phofshoana ea lekhasi e hloekisitsoeng ka mor'a;phofshoana ea lekhasi e hloekisitsoeng ka mor'a;phofshoana ea lekhasi e hloekisitsoeng ka mor'a;PH e fetotsoe ho 3.5 ka glacial acetic acid, e tlhotliloeng ka funele e qhibilihang e otlolohileng, e tiisitsoeng ka pitsa, 'me ea hloekisoa ka mouoane ho 100 ℃ halofo ea hora ho fumana ente ea posterior pituitary.Afterleaf powder [teko ea titer]→[pH3.5] Afterleaf injection [filtration, sterilization]→ ente ea afterleaf e felile sehlahisoa.