PACAP 1-27 CAS: 127317-03-7 pituitary adenylate cyclase activating*polypeptid

Neuropituitrin waa vasoconstrictor iyo antidiuretic, kaas oo loo isticmaali karo dhiig-baxa dhalmada ka dib, kor u qaadida foosha ilmo-galeenka iyo ilmo iska soo rididda aan dhamaystirnayn.Waxa kale oo loo isticmaalaa daawaynta fundus hunguriga iyo gastric fundus varices dhiigbax dilaac, insipidus sonkorowga iyo wixii la mid ah.Hababka wax soo saarka Soo saarista budada caleenta dambe ee pituitary Caleenta dambe ayaa lagu qallajiyey qalajiyaha faakuumka leh oo leh fosfooraska pentoxide (qoyaan ka yar 3%) oo lagu shubay budo leh mashiinka kubbadda si loo helo budada caleenta dambe ee qallafsan.Dabadeed ku dar 90% acetone (3-6 jeer), ku walaaq 25 ℃ 4saac oo ka bax habeen.Sidaa darteed daawaynta soo noqnoqda 2-3 jeer, qaado qadar yar oo dareere acetone ah iyo qadar yar oo biyo nadiif ah oo aan lahayn turbidity.Kadibna waxaa lagu daaweeyaa ether (3 jeer qaddarka) 1 mar, la sifeeyay, ether-dabiiciga dabiiciga ah si ay u qallajiso, oo la helo ka dib budada caleen.Acetone, daawaynta ether waa in meesha laga saaro walxaha molecular hooseeya iyo lipids, si loo hubiyo caddaynta.Porcine neurohypophysial [P2O5]→ budada hypophysial ceriin [acetone, ether] →[25℃,4h] Nadiifinta budada hypophysial Budada hypophysial waxaa la soo saaray laba jeer iyadoo lagu daray 0.25% acetic acid, markii ugu horeysay ee lagu daro 5 jeer qadarka, mar labaad ku dar 2 jeer qadarka, walaaqaya 50 ℃ 15min, sifeynta.Ka dib markii labada soo saarid la dhammeeyay, sifaynta ayaa la isku daray oo lagu kululeeyay xawaarihii Kitaab Kiimiko ilaa 100 ℃ 15min.Kadib 0.5% trichloro-tert-butanol (1: 1 saamiga mugga, daawaynta kaarboonka firfircoon haddii loo baahdo) ayaa lagu daray iyadoo kulul.Sifeynta ayaa lagu qaboojiyey xawaare degdeg ah oo ka hooseeya 30 ℃.Soosaarka acetic acid wuxuu ka kooban yahay borotiinno aan waxtar lahayn iyo enzymes is-hydrolyzing, kuwaas oo la isku dhejin karo laguna burburin karo kuleyliyaha (xusuusnow: oxytocin titer of oxytocin waa sahlan tahay in ay ku fashilmaan hoostooda alkaline ama acids xooggan. Ku xasilan acids daciif ah sida pH3-4, iyo jeermiska uumiga at 100 ℃ 30min ama nadiifinta cadaadis kulul sidoo kale waa mid deggan).budada caleenta post-pituitary [0.25% acetic acid] →[50℃,15min] sifeeyo [0.5% trichloro-tert-butanol]→[la qaboojiyey, la sifeeyey] budada caleen ee la safeeyey ka dib;budada caleenta oo la safeeyey;budada caleenta oo la safeeyey;budada caleenta oo la safeeyey;budada caleenta oo la safeeyey;budada caleenta oo la safeeyey;budada caleenta oo la safeeyey;budada caleenta oo la safeeyey;budada caleenta oo la safeeyey;budada caleenta oo la safeeyey;pH waxaa lagu hagaajiyay 3.5 oo leh glacial acetic acid, lagu sifeeyay funnel dhalaalaysa toosan, dheri-shaabaysan, oo lagu nadiifiyey uumiga 100 ℃ nus saac si loo helo duritaanka dambe ee pituitary.Budada Afterleaf [titer test]→[pH3.5] Cirbad caleen danbe [shaandhayn, jeermis]→ duritaan caleen danbe ah oo dhammaatay.