PACAP 1-27 CAS: 127317-03-7 pituitary adenylate cyclase activating * polypeptid

Neuropituitrin is vasoconstrictor uye antidiuretic, inogona kushandiswa postpartum hemostasis, kukurudzira uterine contractions uye kubvisa pamuviri kusina kukwana.Inoshandiswawo pakurapa esophageal uye gastric fundus varices rupture kubuda ropa, chirwere cheshuga insipidus uye zvichingodaro.Nzira dzekugadzira Kubviswa kwepituitary posterior leaf powder Shizha rekumashure rakaomeswa muvacuum dryer ine phosphorus pentoxide (unyoro huri pasi pe3%) uye ndokukuyiwa kuita upfu nechigayo chebhora kuti pawane hupfu hwakakora hwemashure hwemashizha.Zvadaro wobva wawedzera 90% acetone (3-6 nguva), fambisa pa25 ℃ kwe4h uye uende usiku hwose.Saka inodzokororwa kurapwa 2-3 nguva, tora diki diki yeacetone fluid uye shoma shoma yemvura yakasvibiswa haisisina turbidity.Zvadaro inobatwa ne ether (3 nguva yakawanda) kwe1 nguva, yakasefa, ether natural volatilization kuti iome, inowanikwa mushure mehupfu hwemashizha.Acetone, ether kurapwa ndeyekubvisa yakaderera mamorekuru zvinhu uye lipids, kuve nechokwadi kujeka.Porcine neurohypophysial [P2O5]→ crude hypophysial powder [acetone, ether]→[25℃,4h] Kucheneswa kwehypophysial powder Iyo hypophysial powder yakatorwa kaviri nekuwedzera 0.25% acetic acid, kekutanga kuwedzera 5 nguva yakawanda, kechipiri. kuwedzera 2 nguva yakawanda, ichikurudzira pa 50 ℃ ye 15min, filtration.Mushure mokunge zvinyorwa zviviri zvapera, filtrate yakabatanidzwa uye inopisa nokukurumidza Chemicalbook speed kusvika 100 ℃ ye15min.Zvino 0.5% trichloro-tert-butanol (1: 1 vhoriyamu reshiyo, activated kabhoni kurapwa kana zvichidikanwa) yakawedzerwa ichipisa.Iyo filtrate yakanyoroveswa nekukurumidza kusvika pazasi 30 ℃.Iyo acetic acid inobvisa ine mapuroteni asingashande uye ega-hydrolyzing enzymes, iyo inogona kubatanidzwa uye kuparadzwa nekupisa (chinyorwa: iyo oxytocin titer ye oxytocin iri nyore kukundikana pasi pe alkaline kana yakasimba acids. Yakagadzikana mune isina simba acids se pH3-4, uye steam sterilization pa 100 ℃ ye 30min kana kupisa kudzvinyirirwa sterilization zvakare yakagadzikana).Post-pituitary leaf powder [0.25% acetic acid]→[50℃,15min] filtrate [0.5% trichloro-tert-butanol]→[firiji, yakapepetwa] post-anatswa shizha upfu;post-purified leaf powder;post-purified leaf powder;post-purified leaf powder;post-purified leaf powder;post-purified leaf powder;post-purified leaf powder;post-purified leaf powder;post-purified leaf powder;post-purified leaf powder;Iyo pH yakagadziridzwa kuita 3.5 ine glacial acetic acid, yakasefa neyakamira yakanyunguduka funnel, hari-yakavharwa, uye yakavharwa nemhepo pa100 ℃ kwehafu yeawa kuti uwane posterior pituitary jekiseni.Afterleaf poda [titer test]→[pH3.5] Afterleaf jekiseni [kusefa, sterilization]→ jekiseni releaf rapera chigadzirwa.