PACAP 1-27 CAS: 127317-03-7 pituitary adenylate cyclase activating*polypeptide

Neuropituitrin o se vasoconstrictor ma antidiuretic, lea e mafai ona faʻaaogaina mo hemostasis postpartum, faʻalauteleina le faʻamaʻiina o le uterine ma le le atoatoa o le faapau pepe.E fa'aaogaina fo'i mo togafitiga o le esophageal ma le gastric fundus varices rupture bleeding, ma'i suka insipidus ma isi.Metotia Gaosia Ave'ese o le pa'u laulaau pito i tua O le lau pito i tua sa fa'amago i se masini fa'agogo ma le phosphorus pentoxide (sūsū e itiiti ifo i le 3%) ma fa'apalapala i se pa'u polo e maua ai le pa'u laula'au pito i tua.Ona faaopoopo lea o le 90% acetone (3-6 taimi), faʻafefe i le 25 ℃ mo le 4h ma tuʻu i le po.O lea e toe fai togafitiga 2-3 taimi, ave sina vaega o le acetone vai ma sina vaega itiiti o vai faʻafefe e le toe faʻafefe.Ona togafitia lea i le eter (3 taimi le aofaʻi) mo le 1 taimi, faʻamama, faʻafefe faʻanatura eter e mago, maua pe a uma lau paʻu.Acetone, ether togafitiga o le aveesea o mea maualalo molecular ma lipids, ina ia mautinoa le manino.Porcine neurohypophysial [P2O5]→ crude hypophysial powder [acetone, ether]→ [25℃,4h] Faʻamamaina o le paʻu o le hypophysial O le paʻu o le hypophysial na faʻaluaina e ala i le faʻaopopoina o le 0.25% acetic acid, o le taimi muamua faʻaopoopo 5 taimi le aofaʻi, o le taimi lona lua fa'aopoopo 2 taimi le aofa'i, fa'aoso i le 50 ℃ mo le 15min, filtration.A mae'a fa'ato'aga e lua, ona tu'u fa'atasi lea o le filtrate ma fa'avevela i le saoasaoa o le tusi o vaila'au i le 100 ℃ mo le 15min.Ona faaopoopo lea o le 0.5% trichloro-tert-butanol (1:1 volume ratio, activated carbon treatment pe'ā manaʻomia) aʻo vevela.O le filtrate na fa'alili i se saoasaoa vave i lalo ole 30 ℃.O le acetic acid extract o loʻo i ai polotini le aoga ma enzymes-hydrolyzing, lea e mafai ona faʻamaopoopoina ma faʻaumatia e ala i le faʻamafanafanaina (faʻaaliga: o le oxytocin titer o le oxytocin e faigofie ona paʻu i lalo o le alkaline poʻo le malosi malosi. Stable i mea vaivai e pei ole pH3-4, ma ausa sterilization i le 100 ℃ mo le 30min po o le vevela vevela sterilization foi mautu).Fa'amama lau pa'u pa'u [0.25% acetic acid]→[50℃,15min] filtrate [0.5% trichloro-tert-butanol]→[fa'a'a'a'ai, fa'amamā] fa'amama lau pa'u;fa'amama lau pa'u;fa'amama lau pa'u;fa'amama lau pa'u;fa'amama lau pa'u;fa'amama lau pa'u;fa'amama lau pa'u;fa'amama lau pa'u;fa'amama lau pa'u;fa'amama lau pa'u;O le pH na faʻafetaui i le 3.5 faʻatasi ai ma le acetic acid glacial, faʻamama e ala i luga o le faʻafefete faʻafefeteina, faʻamaufaʻailoga, ma faʻamaʻi i le ausa i le 100 ℃ mo le afa itula e maua ai le tui pito i tua.Afterleaf powder [titer test]→[pH3.5] Afterleaf tui [filtration, sterilization]→ afterleaf tui oloa maeʻa tui.