PACAP 1-27 CAS: 127317-03-7 pituitary adenylate cyclase activating*polypeptid

Neuropituitrin is a vasoconstrictor and antidiuretic, which can be used for postpartum hemostasis, promoting uterine contractions and incomplete abortion. It is also used for the treatment of esophageal and gastric fundus varices rupture bleeding, diabetes insipidus and so on. Production methods Extraction of pituitary posterior leaf powder The posterior leaf was dried in a vacuum dryer with phosphorus pentoxide (moisture less than 3%) and ground into powder with a ball mill to obtain coarse posterior leaf powder. Then add 90% acetone (3-6 times), stir at 25℃ for 4h and leave overnight. So repeated treatment 2-3 times, take a small amount of acetone liquid and a small amount of distilled water no longer turbidity. Then treated with ether (3 times the amount) for 1 time, filtered, ether natural volatilization to dry, obtained after leaf powder. Acetone, ether treatment is to remove low molecular substances and lipids, to ensure clarity. Porcine neurohypophysial [P2O5]→ crude hypophysial powder [acetone, ether]→[25℃,4h] Purification of hypophysial powder The hypophysial powder was extracted twice by adding 0.25% acetic acid, the first time adding 5 times the amount, the second time adding 2 times the amount, stirring at 50℃ for 15min, filtration. After the two extractions were completed, filtrate was combined and heated at a rapid Chemicalbook speed to 100℃ for 15min. Then 0.5% trichloro-tert-butanol (1:1 volume ratio, activated carbon treatment if necessary) was added while hot. The filtrate was cooled at a rapid rate to below 30℃. The acetic acid extract contains ineffective proteins and self-hydrolyzing enzymes, which can be coagulated and destroyed by heating (note: the oxytocin titer of oxytocin is easy to fail under alkaline or strong acids. Stable in weak acids such as pH3-4, and steam sterilization at 100℃ for 30min or hot pressure sterilization is also stable). Post-pituitary leaf powder [0.25% acetic acid]→[50℃,15min] filtrate [0.5% trichloro-tert-butanol]→[refrigerated, filtered] post-purified leaf powder; post-purified leaf powder; post-purified leaf powder; post-purified leaf powder; post-purified leaf powder; post-purified leaf powder; post-purified leaf powder; post-purified leaf powder; post-purified leaf powder; post-purified leaf powder; The pH was adjusted to 3.5 with glacial acetic acid, filtered by vertical melting funnel, pot-sealed, and sterilized by steam at 100℃ for half an hour to obtain posterior pituitary injection. Afterleaf powder [titer test]→[pH3.5] Afterleaf injection [filtration, sterilization]→ afterleaf injection finished product.