PACAP 1-27 CAS: 127317-03-7 pituitary adenylate cyclase activating * polypeptid

Neuropituitrin ndi vasoconstrictor ndi antidiuretic, yomwe ingagwiritsidwe ntchito popanga hemostasis ya postpartum, kulimbikitsa kutsekeka kwa chiberekero ndi kuchotsa mimba kosakwanira.Amagwiritsidwanso ntchito zochizira esophageal ndi chapamimba fundus varices chophukacho magazi, shuga insipidus ndi zina zotero.Njira zopangira Kutulutsa ufa wa pituitary posterior posterior leaf Tsamba lakumbuyo limawumitsidwa mu chowumitsira chowumitsira ndi phosphorous pentoxide (chinyezi chochepera 3%) ndikusiyidwa kukhala ufa ndi mphero kuti apeze ufa wosalala wakumbuyo.Kenako onjezani 90% acetone (nthawi 3-6), yambitsani pa 25 ℃ kwa 4h ndikusiya usiku wonse.Choncho mobwerezabwereza mankhwala 2-3, kutenga pang`ono acetone madzi ndi pang`ono osungunula madzi salinso turbidity.Kenako amathandizidwa ndi etere (kuchuluka kwa katatu) kwa nthawi imodzi, osasefedwa, ether zachilengedwe volatilization kuti ziume, zopezedwa pambuyo pa ufa wa masamba.Acetone, chithandizo cha ether ndikuchotsa zinthu zochepa zama cell ndi lipids, kuwonetsetsa kumveka bwino.Porcine neurohypophysial [P2O5]→ crude hypophysial powder [acetone, ether]→[25℃,4h] Kuyeretsedwa kwa hypophysial powder The hypophysial powder inatulutsidwa kawiri powonjezera 0.25% acetic acid, nthawi yoyamba kuwonjezera nthawi 5 kuchuluka kwake, kachiwiri. kuwonjezera 2 nthawi kuchuluka, oyambitsa pa 50 ℃ kwa 15min, kusefera.Pambuyo pazigawo ziwirizi, kusefa kunaphatikizidwa ndikutenthedwa pa liwiro la Chemicalbook mpaka 100 ℃ kwa 15min.Kenako 0,5% trichloro-tert-butanol (1: 1 voliyumu chiŵerengero, adamulowetsa mpweya mankhwala ngati n'koyenera) anawonjezera kutentha.Filtrate idakhazikika mwachangu mpaka pansi pa 30 ℃.The acetic acid Tingafinye muli mapuloteni osagwira ntchito ndi self-hydrolyzing michere, amene akhoza coagulated ndi kuwonongedwa ndi kutentha (chidziwitso: oxytocin titer ya oxytocin ndi yosavuta kulephera pansi zamchere kapena amphamvu zidulo. Kukhazikika mu zidulo ofooka monga pH3-4, ndi kutsekereza kwa nthunzi pa 100 ℃ kwa 30min kapena kutentha kwapakati pa kutentha kumakhalanso kokhazikika).Ufa wa masamba a post-pituitary [0.25% acetic acid]→[50℃,15min] kusefera [0.5% trichloro-tert-butanol]→[wosefedwa mufiriji] woyeretsedwa pambuyo pake;ufa wa masamba oyeretsedwa pambuyo pake;ufa wa masamba oyeretsedwa pambuyo pake;ufa wa masamba oyeretsedwa pambuyo pake;ufa wa masamba oyeretsedwa pambuyo pake;ufa wa masamba oyeretsedwa pambuyo pake;ufa wa masamba oyeretsedwa pambuyo pake;ufa wa masamba oyeretsedwa pambuyo pake;ufa wa masamba oyeretsedwa pambuyo pake;ufa wa masamba oyeretsedwa pambuyo pake;PH idasinthidwa kukhala 3.5 ndi glacial acetic acid, yosefedwa ndi fungulo losungunuka loyima, lotsekedwa ndi mphika, ndikutsukidwa ndi nthunzi pa 100 ℃ kwa theka la ola kuti alandire jekeseni wapambuyo wa pituitary.Afterleaf ufa [titer test]→[pH3.5] Afterleaf jakisoni [sefera, yotseketsa]→ jakisoni wa afterleaf watha mankhwala.