PACAP 1-27 CAS: 127317-03-7 pituitary adenylate cyclase whakahohe*polypeptid

Ko te Neuropituitrin he vasoconstrictor me te antidiuretic, ka taea te whakamahi mo te hemostasis postpartum, te whakatairanga i nga whakawhitinga uterine me te materoto kaore i oti.Ka whakamahia hoki mo te maimoatanga o te esophageal me te gastric fundus varices rupture bleeding, te mate huka insipidus me etahi atu.Nga tikanga whakaputa Te tangohanga o te paura rau i muri i te pituitary I whakamarokehia te rau o muri i roto i te whakamaroke korehau me te phosphorus pentoxide (te makuku iti iho i te 3%), ka whakamaroke ki te paura me te mira poroporo kia whiwhi ai i te paura rau o muri.Kātahi ka tāpirihia te 90% acetone (3-6 wa), whakaohokia i te 25 ℃ mo te 4 haora ka waiho mo te po.Na ka tukuruatia te maimoatanga kia 2-3 nga wa, tangohia he wai iti acetone me te iti o te wai maturu kia kore e pupuhi.Na ka tukinotia ki te ether (e toru nga wa te nui) mo te 1 wa, ka tātarihia, ka whakamarokehia te ether kia maroke, ka whiwhi i muri i te paura rau.Acetone, ether maimoatanga he ki te tango iti matū rāpoi me lipids, ki te whakarite mārama.Porcine neurohypophysial [P2O5]→ paura hypophysial crude [acetone, ether]→ [25℃,4h] Te purenga o te paura hypophysial Ko te paura hypophysial i tangohia e rua ma te taapiri i te 0.25% waikawa acetic, ko te wa tuatahi ka taapirihia e 5 nga wa te nui, te wa tuarua tāpirihia kia 2 nga wa te nui, te whakaoho i te 50 ℃ mo te 15min, te tātari.I muri i te otinga o nga tangohanga e rua, ka whakakotahihia te filtrate me te whakamahana i te tere pukapuka Chemical ki te 100 ℃ mo te 15min.Kātahi ka tāpirihia te 0.5% trichloro-tert-butanol (1:1 ōwehenga rōrahi, te maimoatanga waro whakahohe mēnā e tika ana) i te wā e wera ana.I whakamataohia te filtrate i te tere tere ki raro i te 30 ℃.Kei roto i te tangohanga waikawa acetic he pūmua kore whai hua me te whaiaro-hydrolyzing whākōkī, e taea te coagulated me te whakangaro i te whakawera (note: te oxytocin titer o te oxytocin he ngawari ki te kore i raro i te waikawa kawakore, waikawa kaha ranei. E mau ana i roto i nga waikawa ngoikore penei i te pH3-4, me te whakahoromatatanga mamaoa i te 100 ℃ mo te 30min, kei te pumau ano hoki te whakahorotanga pehanga wera).Te paura rau muri-pituitary [0.25% waikawa acetic]→[50℃,15min] filtrate [0.5% trichloro-tert-butanol]→[mao, tātari] paura rau i muri i purea;paura rau i muri i te purea;paura rau i muri i te purea;paura rau i muri i te purea;paura rau i muri i te purea;paura rau i muri i te purea;paura rau i muri i te purea;paura rau i muri i te purea;paura rau i muri i te purea;paura rau i muri i te purea;I whakatikatikahia te pH ki te 3.5 me te waikawa acetic glacial, ka tātarihia e te kohanga whakarewa poutū, ka hirihia te kohua, ka whakahoroa e te mamaoa i te 100 ℃ mo te hawhe haora kia whiwhi i te werohanga pituitary o muri.Te paura o muri [whakamātautau titer]→[pH3.5] Te werohanga rau muri [te tātari, te whakahoromata]→ te hua kua oti te werohanga rau.