PACAP 1-27 CAS: 127317-03-7 pituitary adenylate cyclase na-arụ ọrụ * polypeptid

Neuropituitrin bụ vasoconstrictor na antidiuretic, nke enwere ike iji maka hemostasis mgbe ime nwa, na-akwalite mmechi nke akpanwa na ite ime na-ezughị ezu.A na-ejikwa ya maka ọgwụgwọ nke esophageal na gastric fundus varices mgbawa ọbara ọgbụgba, ọrịa shuga insipidus na ndị ọzọ.Ụzọ mmepụta Mwepụta nke pituitary posterior akwukwo ntụ ntụ Akpọrọ akwụkwọ n'azụ n'ime igwe na-ekpo ọkụ nwere phosphorus pentoxide (mmiri na-erughị 3%) wee gwerie ya n'ime ntụ ntụ na igwe igwe bọọlụ iji nweta ntụ ntụ dị n'azụ.Mgbe ahụ tinye 90% acetone (ugboro 3-6), kpalie na 25 ℃ maka awa 4 wee pụọ n'abali.Ya mere ọgwụgwọ ugboro ugboro ugboro 2-3, were obere mmiri mmiri acetone na obere mmiri a na-ekpo ọkụ na-abaghị uru.Mgbe ahụ, a na-emeso ya na ether (ugboro 3 ego) maka oge 1, a na-ehichapụ, ether eke volatilization ka nkụ, nwetara mgbe akwukwo ntụ ntụ.Acetone, ọgwụgwọ ether bụ iwepu ihe ndị dị ala na lipids, iji hụ na idoanya.Porcine neurohypophysial [P2O5]→ crude hypophysial ntụ ntụ [acetone, ether] →[25℃,4h] Ịdị ọcha nke ntụ ntụ ntụ ntụ ntụ ntụ ntụ ntụ ntụ a na-ewepụta ugboro abụọ site n'ịgbakwunye 0.25% acetic acid, nke mbụ na-agbakwunye 5 ugboro ego, nke abụọ oge. na-agbakwunye 2 ugboro ego, edemede na 50 ℃ maka 15min, filtration.Ka emechara mmịpụta abụọ ahụ, a na-ejikọta filtrate wee kpoo ọkụ na ngwa ngwa Chemicalbook ruo 100 ℃ maka nkeji iri na ise.Mgbe ahụ 0,5% trichloro-tert-butanol (1: 1 nha nha, ọgwụgwọ carbon arụ ọrụ ma ọ bụrụ na ọ dị mkpa) agbakwunyere mgbe ọ na-ekpo ọkụ.A jụrụ filtrate n'ọsọ ọsọ ruo n'okpuru 30 ℃.Ngwunye acetic acid nwere protein na-adịghị arụ ọrụ na enzymes nke na-eme onwe ya, nke nwere ike ịchịkọta ma bibie site na ikpo ọkụ (rịba ama: oxytocin titer nke oxytocin dị mfe ịdaba n'okpuru alkaline ma ọ bụ acid siri ike. Stable na acid na-adịghị ike dị ka pH3-4, na uzuoku igba ogwu na 100 ℃ maka 30min ma ọ bụ na-ekpo ọkụ sterilization bụkwa kwụsiri ike).Post-pituitary leaf ntụ ntụ [0.25% acetic acid]→[50℃,15min] filtrate [0.5% trichloro-tert-butanol]→[refrigerated, filtered] post-ọcha akwukwo ntụ ntụ;akwụkwọ ntụ ntụ ntụ ọcha nke post-ọcha;akwụkwọ ntụ ntụ ntụ ọcha nke post-ọcha;akwụkwọ ntụ ntụ ntụ ọcha nke post-ọcha;akwụkwọ ntụ ntụ ntụ ọcha nke post-ọcha;akwụkwọ ntụ ntụ ntụ ọcha nke post-ọcha;akwụkwọ ntụ ntụ ntụ ọcha nke post-ọcha;akwụkwọ ntụ ntụ ntụ ọcha nke post-ọcha;akwụkwọ ntụ ntụ ntụ ọcha nke post-ọcha;akwụkwọ ntụ ntụ ntụ ọcha nke post-ọcha;A gbanwere pH ahụ ka ọ bụrụ 3.5 jiri glacial acetic acid, nke a na-agbaze site na oghere agbaze kwụ ọtọ, nke ejiri ite mechie, wee mee ka uzuoku dị 100 ℃ mee ka ọ bụrụ ọkara elekere maka ọkara elekere iji nweta ntụtụ pituitary azụ.Afterleaf ntụ ntụ [titer test]→[pH3.5] Afterleaf injection [filtration, sterilization]→ afterleaf injection okokụre ngwaahịa.