PACAP 1-27 CAS: 127317-03-7 pituitary adenylate cyclase activating * polypeptid

Neuropituitrin yog vasoconstrictor thiab antidiuretic, uas tuaj yeem siv rau tom qab hemostasis, txhawb nqa uterine contractions thiab rho menyuam tsis tiav.Nws kuj yog siv rau kev kho mob ntawm esophageal thiab gastric fundus varices rupture los ntshav, ntshav qab zib insipidus thiab hais txog.Cov txheej txheem ntau lawm Extraction ntawm pituitary posterior nplooj nplooj hmoov Cov nplooj ntawv tom qab tau qhuav hauv lub tshuab nqus tsev nrog phosphorus pentoxide (qe noo tsawg dua 3%) thiab hauv av rau hauv cov hmoov nrog lub pob zeb kom tau cov hmoov tawv tom qab nplooj.Tom qab ntawd ntxiv 90% acetone (3-6 zaug), do ntawm 25 ℃ rau 4h thiab tawm thaum hmo ntuj.Yog li rov kho dua 2-3 zaug, noj me me ntawm acetone kua thiab me me ntawm cov dej distilled tsis muaj turbidity.Tom qab ntawd kho nrog ether (3 zaug tus nqi) rau 1 zaug, lim, ether ntuj volatilization kom qhuav, tau tom qab nplooj hmoov.Acetone, ether kev kho mob yog tshem tawm cov tshuaj tsis muaj molecular thiab lipids, kom paub meej.Porcine neurohypophysial [P2O5] → crude hypophysial hmoov [acetone, ether] → [25 ℃, 4h] Purification ntawm hypophysial hmoov Cov hmoov hypophysial tau muab rho tawm ob zaug los ntawm kev ntxiv 0.25% acetic acid, thawj zaug ntxiv 5 zaug tus nqi, zaum thib ob ntxiv 2 zaug ntawm tus nqi, nplawm ntawm 50 ℃ rau 15 feeb, pom.Tom qab ob qhov kev rho tawm tiav lawm, lim tau ua ke thiab ua kom sov ntawm cov ntaub ntawv ceev ceev rau 100 ℃ rau 15 feeb.Tom qab ntawd 0.5% trichloro-tert-butanol (1: 1 ntim piv, activated carbon yog tsim nyog) tau ntxiv thaum kub.Cov lim tau txias ntawm tus nqi nrawm kom qis dua 30 ℃.Cov acetic acid extract muaj cov proteins uas tsis muaj txiaj ntsig thiab nws tus kheej-hydrolyzing enzymes, uas tuaj yeem coagulated thiab rhuav tshem los ntawm cua sov (ceeb toom: oxytocin titer ntawm oxytocin yog ib qho yooj yim ua tsis tau raws li alkaline lossis muaj zog acids, ruaj khov hauv cov acids tsis muaj zog xws li pH3-4, thiab chav sterilization ntawm 100 ℃ rau 30min los yog kub siab sterilization kuj ruaj khov).Post-pituitary nplooj hmoov [0.25% acetic acid] → [50 ℃, 15min] filtrate [0.5% trichloro-tert-butanol] → [tub yees, lim] post-purified nplooj hmoov;post-purified nplooj hmoov;post-purified nplooj hmoov;post-purified nplooj hmoov;post-purified nplooj hmoov;post-purified nplooj hmoov;post-purified nplooj hmoov;post-purified nplooj hmoov;post-purified nplooj hmoov;post-purified nplooj hmoov;Cov pH tau hloov kho mus rau 3.5 nrog glacial acetic acid, lim los ntawm ntsug melting funnel, lub lauj kaub kaw, thiab sterilized los ntawm chav ntawm 100 ℃ rau ib nrab ib teev kom tau txais kev txhaj tshuaj tom qab pituitary.Afterleaf hmoov [titer test] → [pH3.5] Afterleaf txhaj [filtration, sterilization] → afterleaf txhaj cov khoom tiav.