PACAP 1-27 CAS: 127317-03-7 pituitary adenylate cyclase kunnawa * polypeptid

Neuropituitrin ne vasoconstrictor da antidiuretic, wanda za a iya amfani da bayan haihuwa hemostasis, inganta igiyar ciki contractions da kuma rashin cikakken zubar da ciki.Ana kuma amfani da shi don maganin cututtukan fundus na esophageal da na ciki varices fashewar zubar jini, ciwon sukari insipidus da sauransu.Hanyoyin samarwa Ana fitar da foda na baya na pituitary leaf na baya an bushe a cikin injin bushewa tare da phosphoric pentoxide (danshi kasa da 3%) da ƙasa a cikin foda tare da injin niƙa don samun foda mai laushi na baya.Sa'an nan kuma ƙara 90% acetone (sau 3-6), motsawa a 25 ℃ na 4h kuma bar dare.Don haka maimaita magani sau 2-3, ɗauki ƙaramin adadin ruwa na acetone da ƙaramin adadin ruwan da ba za a iya jurewa ba.Sa'an nan kuma bi da ether (sau 3 adadin) don 1 lokaci, tacewa, ether na halitta volatilization zuwa bushe, samu bayan leaf foda.Acetone, ether magani shine cire ƙananan ƙwayoyin ƙwayoyin cuta da lipids, don tabbatar da tsabta.Porcine neurohypophysial [P2O5]→ danyen hypophysial foda [acetone, ether] →[25 ℃, 4h] tsarkakewa na hypophysial foda An fitar da foda hypophysial sau biyu ta ƙara 0.25% acetic acid, karo na farko yana ƙara 5 sau adadin, karo na biyu. ƙara sau 2 adadin, yana motsawa a 50 ℃ na 15min, tacewa.Bayan da aka kammala hakar guda biyu, an haɗa tacewa kuma an yi zafi a cikin saurin littafin Chemical zuwa 100 ℃ na 15min.Sa'an nan 0.5% trichloro-tert-butanol (1: 1 girma rabo, kunna carbon jiyya idan ya cancanta) da aka kara yayin zafi.An sanyaya tacewar a cikin sauri zuwa ƙasa da 30 ℃.Acetic acid tsantsa ya ƙunshi sunadarai marasa tasiri da kuma enzymes masu sarrafa kansu, wanda za'a iya hadewa da lalata ta hanyar dumama (bayanin kula: oxytocin titer na oxytocin yana da sauƙi a kasa a karkashin alkaline ko acid mai karfi. Stable a cikin raunin acid kamar pH3-4, da kuma tururi bakara a 100 ℃ na 30min ko zafi haifuwa ne kuma barga).Post-pituitary leaf foda [0.25% acetic acid] →[50 ℃,15min] tacewa [0.5% trichloro-tert-butanol] →[mai firiji, tacewa] foda leaf mai tsabta;foda ganye mai tsabta;foda ganye mai tsabta;foda ganye mai tsabta;foda ganye mai tsabta;foda ganye mai tsabta;foda ganye mai tsabta;foda ganye mai tsabta;foda ganye mai tsabta;foda ganye mai tsabta;An daidaita pH zuwa 3.5 tare da glacial acetic acid, tace ta hanyar narkewar mazurari a tsaye, a rufe tukunya, kuma haifuwa ta tururi a 100 ℃ na rabin sa'a don samun allurar pituitary na baya.Bayan leaf foda [titer gwajin] →[pH3.5] Afterleaf allura [tace, bakara]→ afterleaf allura gama samfurin.